It will be Garcia’s first missed major since the 1999 US Open.
“After 21 years of not missing a Major Championship, I will sadly miss The Masters this week,” the Spaniard wrote on Twitter on Monday.
“The important thing is that my family and I are feeling good. We’ll come back stronger and give the green jacket a go next April.
“On Saturday night after driving back from the Houston Open, I started feeling a bit of a sore throat and a cough. The symptoms stayed with me on Sunday morning so I decided to get tested for COVID-19 and so did my wife Angela. Thankfully she tested negative, but I didn’t.”
The 2020 Masters, which was postponed from it’s usual April slot because of the coronavirus pandemic, will begin on Thursday.
Garcia last played in the first two rounds of the Houston Open last weekend. He failed to make the cut.
The event was the first on the PGA Tour in the US to allow fans back on-site.